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    NIH - Funded Training in Cell Signaling & Lung Pathobiology
    		 
     
    Kathy M. Bonness
    Phone: (251) 460-6861
    Fax: (251) 460-6402
    Email: kbonness@hotmail.com
     
    EDUCATION AND QUALIFICATIONS
    2002-2008 PhD Candidate
      Biochemistry and Molecular Biology (Center for Lung Biology)
      University of South Alabama
      Mobile, AL
      Advisor: Richard E. Honkanen, Ph.D.
       
    1995-1997  Pre-medical Studies
      Non-degree Seeking
      University of South Alabama
      Mobile, AL
      Advisor:  Dr. T.G. Jackson.  (President’s List: 1995/1996)
       
    1988-1992 Bachelor of Arts
      Political Science
      Santa Clara University
      Santa Clara, CA
      Advisor: Dr. William Stover (Dean’s List 1990-1991)
       
    HONORS AND AWARDS
    2004-2007 Institutional pre-doctoral trainee in Cell Signaling and Lung Pathobiology (2004-07)
      NIH Training Grant T32 HL076125
      (Center for Lung Biology) University of South Alabama, Mobile, AL
       
    2003-2006   Hypothesis-drive microscopy images exhibited by PERCIPIOTM: Mobile Museum of Art; The Gulf Coast Emporium Science Center
      USA Library; and PERCIPIOTM promotional video


    PUBLICATIONS

    Peer-Reviewed Research Articles

    1.  Bonness KM, Dean N, Honkanen RE.  Fostriecin Inhibition of PP4: Anti-Proliferative Properties of Fostriecin due to Mitotic Arrest and Cytokinesis Failure Resulting from Suppression of PP4. (manuscript in preparation)

    2.  Bonness KM, Aragon IV, Rutland B, Ofori-Acquah S, Dean ND, and Honkanen RE. Cantharidin-Induced Mitotic Arrest is Associated with the Formation of Aberrant Mitotic Spindles and Lagging Chromosomes Resulting, in part, from the Suppression of PP2Aa.  Mol Cancer Ther 5, 2727-2736, 2006.

    3.  Buck SB, Hardouin C, Ichikawa S, Soenen DR, Gauss C-M, Hwang I, Swingle M, Bonness KM, Honkanen RE and Boger DL.  Fundamental Role of the Fostriecin Unsaturated Lactone and Implications for Selective Protein Phosphatase Inhibition. J. Am. Chem. Soc., 125 (51), 15694-15695, 2003.
     
    Abstracts:

    1. D. Skarra, K. Bonness, I. Aragon, T. Golden, B. Rutland, and R. E. onkanen. “Tautomycetin and fostriecin-induced mitotic arrest is associated with the formation of aberrant mitotic spindles and lagging chromosomes, resulting in part from the suppression of PP2Aa”.  FASEB Summer Research Conference on Protein Phosphatases (P91)

    2. Bonness KM, Rutland B, Dean N, Honkanen RE. Prolonged Mitotic Arrest Induced by Cantharidin treatment is Associated with the Formation of Aberrant Mitotic Features Resulting, in part, from the Suppression of PP2A Activity in Human cells. USA Research Day (P91) 2007

    3. Bourgeois A, Swingle M, Bonness KM, Honkanen RE.  Screening of Natural Products and Synthetic Cantharidin Derivatives for PP5 Inhibition via DiFMUP Assay and Epifluorescence Cell Imagery. Medical Student Research Day 2007

    4. Bonness KM, Aragon IV, Dean ND, Honkanen RE.  Cantharidin induced Mitotic Arrest is Associated with the Formation of Aberrant Mitotic Spindles Resulting, in part, from the Suppression of PP2A-alpha Activity in Human Lung Adenocarcinoma cells. ASCB (951/B122) 2005

    5. Bonness KM, Rutland B, Dean N, Honkanen RE. Cantharidin-Induced Mitotic Arrest and Subsequent Apoptosis is Associated with the Formation of Aberrant Mitotic Spindles and Improperly Aligned Chromosomes Resulting, in Part, from the Suppression of PP2Aα Activity in Human Cells. ASCB (87B/95) 2002

    6. Bonness KM, Honkanen RE. Exploring the Molecular Mechanism associated with the Anti-tumor activity of Cantharidin. FASEB Summer Research Conference: Protein Phosphatases, Snowmass, CO. p.16 (2002).

    7. Hicks S, Bonness KM, Honkanen RH. Aberrant Mitotic Spindle Formation in A549 Cells Treated with Cantharidin and Okadaic Acid. Medical Student Research Day (P1)2001

    8. Bonness K, Durcova I, Capley G, Stevens T, Gillespie MN, and Olson JW.  Thrombin Stimulates Sub-cellular Trafficking of Active Ornithine-Decarboxylase in Rat Pulmonary Endothelial Cells. FASEB J., 14:A692,2000.

     
    RESEARCH EXPERIENCE
    8/02-current  Graduate Research Assistant, Department of Biochemistry and Molecular Biology (Center for Lung Biology 2004-2007), University of South Alabama, College of Medicine, Mobile, AL.
      Supervisor: Dr. Richard E. Honkanen
    Project: Examining the role of serine/threonine protein phosphatases in mitosis, specifically to explore the molecular mechanisms correlating the inhibition of serine/threonine phosphatases (S/T PPases) with anti-proliferative effects specifically during mitosis in human cancer cells.
       
    8/00-8/02 Research Technologist III, Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, Mobile, AL. Supervisor:  Dr. Richard E. Honkanen
      Project: Examining the biological and cellular effects of serine/threonine protein phosphatase inhibition by natural product inhibitors and antisense-mediated selective inhibition in human cancer cells.
       
    2/99-7/00 Research Technologist II, Department of Pharmacology, University of South Alabama, College of Medicine, Mobile, AL.  Supervisor: Dr. Jack W. Olson.
      Project:  Examining thrombin-induced subcellular trafficking of ornithine-decarboxylase in rat pulmonary endothelial cells.
       
    1/98-2/99 Research Technician II, Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, Mobile, AL.  Supervisor:  Dr. Steven J. Pittler.
      Project:  Determining the binding region of Crx within the cGMP Phosphodiesterase a-subunit gene promoter required for full transcriptional activity.
       
    9/95-9/97 Assistant Lab Technician, TAI Environmental Sciences, INC., Mobile, AL
      Duties:  Testing client effluent samples according to governmentally regulated standards including chemical and bioassay analysis.
       
    8/96-9/97 Phlebotomist, University of South Alabama Medical Center, Mobile, AL
      Duties:  Blood sample collection; training new employees; proficient in Cerner management collection systems.
       
    95-97  Internships (CP-200), University of South Alabama, Mobile, AL
    Burn Unit, USA Medical Center, Mobile, AL
    Surgery, USA Medical Center, Mobile, AL
    Emergency Room, USA Medical Center, Mobile, AL
     

    Internal Medicine (Osteopath), Knollwood Hospital, Mobile, AL

     
    TEACHING/PRESENTING EXPERIENCE

    Teaching/Invited Speaking Experience:

    12/08  Invited Speaker, St. Mary’s, Honors Science Class, Portland, OR. Targeting Mitosis One Phosphatase at a Time.
    8/06 & 8/07 Invited Speaker, Medical/Scientific Research and the PhD program, SUMMER SCRUBS, University of South Alabama, Mobile, AL. Research in Basic Medical Sciences Targeting Mitosis through Natural Product Inhibition of S/T Phosphatases..
    2007 Medical Student Research Mentor, College of Medicine Summer Research Program, University of South Alabama College of Medicine, Mobile, AL; Austin C. Bourgeois
    2001 Medical Student Research Mentor, College of Medicine Summer Research Program, University of South Alabama College of Medicine, Mobile, AL; Suzanne Hicks.
    2000-2001  Instructor, “Patient Education in Chronic Migraine”, Department of Neurology, USA Knollwood, Mobile, AL. Supervisor:  Dr. John F. Rothrock, MD.  Funding: South Alabama Medical Science Foundation through GlaxoSmithKline unrestricted educational grant.
    1999-2001 Invited Patient Guest Speaker, Department of Neurology, USA Knollwood, Mobile, AL.  Dr. John F. Rothrock.  Sponsored by pharmaceutical companies including: Merck, Abbott, and GlaxoSmithkline Beecham.
       

    Scientific Presentations:
    2003-current Presenter at Research Seminar series, Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, AL
    2004-2007 Presenter at the Research in Progress series, Center for Lung Biology, University of South Alabama, Mobile, AL
    2004-2005  Presenter at Research Seminar series, Department of Cell Biology and, Neuroscience., University of South Alabama, Mobile, AL
       
    PROFESSIONAL ACADEMIC AFFILIATIONS

    2002-Current   Student Member, American Society for Cell Biology
     
    ADDITIONAL SKILLS/EXPERIENCE
    Experimental/Techniques:

    In-Vitro/Molecular and Cellular Biology: Tissue Culture, FACS Analysis, Western/Northern Analysis, RNA, DNA and Protein Isolation, DNA Sequencing, PCR, Anti-sense oligonucleotide mediate suppression, Mutagenesis/Cloning/Transient Transfection, Protein Phosphatase Assay/Inhibitor Screening, Immunocytochemistry/Immunofluorescence, Confocal Microscopy, and Time-lapse Live Cell Video Microscopy.
     

    Imaging and Software:

    Leica TCS SP2 Confocal System processed with Leica Confocal Imaging Software
    Eclipse Nikon TE 2000-U microscope (Neuve live cell chamber/COOLSNAP ES monochrome camera) processed with Metamorph Premier Software and/or NIS Elements
    Endnote and MS Office Programs
     

    Certifications: Radiation and OSHA Biosafety Training
     

    Workshops:

    Grants and Grantsmanship sponsored by the Office of Faculty Affairs and BMS Graduate Office(12/05).
    Careers and Collaborations with Industry sponsored by Office of Faculty Affairs and Department of Physiology (3/07).
    (I/UCRC) Industry/University Cooperative Research Centers. Dr. Denis Gray, strategist/consultant/evaluator to NSF on capturing successful sustainable collaborations (1/08).
     

    Additional Professional Experience:
    8/94-12/94 Legal Assistant, Diammond, Hasser and Frost, Esq., Mobile, AL
    6/94-8/94 Assistant Resident Director, Spring Hill College, Mobile, AL
    7/93-7/94 Trail Coordinator, Mobile County District Attorney’s Office, Strickland Youth Center, Mobile, AL
    3/93-7/93 Clerk, Mobile County District Attorney’s Office, Grand Jury Annex, Mobile, AL
    8/90-7/91 Resident Assistant, Santa Clara University, Santa Clara, CA
    1990-1992  Personal tutor/counselor of young adult at-risk youth. San Jose, CA
       

    Volunteer Experience:

    Amnesty International; 3rd World Child Sponsor Coordinator; Earthquake Relief; Habitat for Humanity; Innvision Julian Street Homeless Shelter for Access I Mentally Ill; Campus Ministry Retreat Organizer; Elderly Shut-In Visitation; Summer Volunteer in Mexico (Service for Smiles); Safety Walk-Along Coordinator; and Salvation Army Soup Kitchen.
     
    Last Update: Aug. 07, 2008