Table 3.  Size of Left Ventricle, Risk Region, and Infarct

 

Heart

LV

Risk Region

Infarct

Risk Region

Infarct

Infarct

 

(mg)

(mg)

(mg)

(mg)

(% of LV)

(% of risk region)

(% of LV)

 

 

 

 

 

 

 

 

Group I

209.4±6.1

148.4±5.6

46.9±4.1

24.0±2.7§=

31.6±2.7

50.9±2.6==

16.0±1.5*#

Group II

153.7±4.9

110.9±4.2

45.3±3.3

24.2±2.5§=

41.0±2.9

53.2±3.6==

22.1±2.4*#

Group III

159.5±9.4

116.5±7.9

50.4±4.0

28.5±2.8§=

43.8±3.1

56.7±2.4==

24.6±2.1*#

Group IV

144.5±4.1

105.7±2.6

51.4±3.7

17.7±1.5*#

49.3±4.0

14.2±1.9*#

17.4±1.5*#

Group V

195.1±6.3

130.6±4.3

46.3±4.0

23.8±3.3=§

35.8±3.0

49.4±3.4=§

18.0±2.3=§

Group VI

192.7±8.7

132.9±4.7

49.9±5.3

11.1±1.9=

38.4±4.2

22.2±2.6=

18.7±1.6=§

Group VII

141.5±7.2

101.4±5.8

40.0±5.7

20.2±3.3=§

39.3±4.7

49.9±4.0=§

19.9±3.1=§

#

Group VIII

159.6±5.6

115.7±4.1

48.3±4.2

11.5±1.4#§

41.8±3.4

25.9±3.3#§=

10.0±1.0#§#

 

Mice in group I (control group, 4-h reperfusion; n=9) underwent a 30-min coronary occlusion followed by 4 h of reperfusion.  Mice in group II (control group, 24-h reperfusion; n=14) underwent a 30-min coronary occlusion followed by 24 h of reperfusion.  In group III (early PC sham group, n=11), the chest was opened for 1 h before a 30-min coronary occlusion followed by 24 h of reperfusion (the 1 h of open-chest state corresponded to the time interval necessary to perform the six occlusion/reperfusion cycles in group IV).  Mice in group IV (early PC group, n=12) were preconditioned with a sequence of six 4-min coronary occlusion /4-min reperfusion cycles; 10 min later, they underwent a 30-min coronary occlusion followed by 24 h of reperfusion.  Mice in groups V and VII (late PC sham group, 4-h and 24-h reperfusion; n=13 and 6, respectively) underwent a thoracotomy and 1 h of open-chest state (without coronary occlusion) on day 1 (the 1 h of open-chest state corresponded to the time interval necessary to perform the six occlusion/reperfusion cycles in groups VI and VIII); 24 h later (on day 2), they underwent a 30-min occlusion followed by 4 h (group V) or 24 h (group VII) of reperfusion.  Mice in group VI (late PC group, 4-h reperfusion; n=14) were preconditioned with a sequence of six 4-min occlusion/4-min reperfusion cycles on day 1; on day 2, they were subjected to a 30-min occlusion followed by 4 h of reperfusion.  Mice in group VIII (late PC group, 24-h reperfusion; n=13) were preconditioned with a sequence of six 4-min occlusion/4-min reperfusion cycles on day 1; on day 2, they underwent a 30-min coronary occlusion followed by 24 h of reperfusion.  LV, left ventricle; Heart, total heart weight (ventricles and atria).  Data are means ± SEM. 

 

*P<0.05 vs. group III; #P<0.05 vs. group II; =P<0.05 vs. group V; P<0.05 vs. group I; §P<0.05 vs. group VII.

 

Reproduced from Ref. 2 with permission.